protein arrays Search Results


pip array pip array  (Echelon Biosciences)
94
Echelon Biosciences pip array pip array
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Pip Array Pip Array, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/pmc11283293-549-0-5?v=Echelon+Biosciences
Average 94 stars, based on 1 article reviews
pip array pip array - by Bioz Stars, 2026-07
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proteon xpr36 protein interaction array system  (Bio-Rad)
96
Bio-Rad proteon xpr36 protein interaction array system
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Proteon Xpr36 Protein Interaction Array System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/us09120875-1787-16-22?v=Bio-Rad
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proteon xpr36 protein interaction array system - by Bioz Stars, 2026-07
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peptide arrays of the ha protein of human influenza virus strains a/california/04/ 2009  (BEI Resources)
90
BEI Resources peptide arrays of the ha protein of human influenza virus strains a/california/04/ 2009
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Peptide Arrays Of The Ha Protein Of Human Influenza Virus Strains A/California/04/ 2009, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/pm40301303-375-4-14?v=BEI+Resources
Average 90 stars, based on 1 article reviews
peptide arrays of the ha protein of human influenza virus strains a/california/04/ 2009 - by Bioz Stars, 2026-07
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protein array of human astrocytomas  (BioChain Institute)
90
BioChain Institute protein array of human astrocytomas
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Protein Array Of Human Astrocytomas, supplied by BioChain Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/pmc02409225-259-4-8?v=BioChain+Institute
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protein array of human astrocytomas - by Bioz Stars, 2026-07
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cytometric bead array human soluble protein kit  (Becton Dickinson)
90
Becton Dickinson cytometric bead array human soluble protein kit
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Cytometric Bead Array Human Soluble Protein Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/pmc06168974-172-20-27?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
cytometric bead array human soluble protein kit - by Bioz Stars, 2026-07
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protein arrays  (imaGenes GmbH)
90
imaGenes GmbH protein arrays
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Protein Arrays, supplied by imaGenes GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/pmc03251905-47-6-8?v=imaGenes+GmbH
Average 90 stars, based on 1 article reviews
protein arrays - by Bioz Stars, 2026-07
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cytoskeletal protein array  (Full Moon BioSystems)
90
Full Moon BioSystems cytoskeletal protein array
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Cytoskeletal Protein Array, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/pm34769418-102-23-26?v=Full+Moon+BioSystems
Average 90 stars, based on 1 article reviews
cytoskeletal protein array - by Bioz Stars, 2026-07
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protein and peptide arrays  (Biomol GmbH)
90
Biomol GmbH protein and peptide arrays
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Protein And Peptide Arrays, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/10__1016_slash_j__trivac__2016__04__003-756-7-18?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
protein and peptide arrays - by Bioz Stars, 2026-07
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protein array kits  (SYGNIS AG)
90
SYGNIS AG protein array kits
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Protein Array Kits, supplied by SYGNIS AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/10__1021_slash_pr0307366-32-16-0?v=SYGNIS+AG
Average 90 stars, based on 1 article reviews
protein array kits - by Bioz Stars, 2026-07
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protein chip array  (CapitalBio Corporation)
90
CapitalBio Corporation protein chip array
(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) <t>PIP</t> <t>array</t> spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.
Protein Chip Array, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/10__1161_slash_hypertensionaha__120__15016-49-0-6?v=CapitalBio+Corporation
Average 90 stars, based on 1 article reviews
protein chip array - by Bioz Stars, 2026-07
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raybio quantibody custom array  (RayBiotech inc)
90
RayBiotech inc raybio quantibody custom array
The expression levels of cytokines marked changes in high myopic patients with cataracts and non-myopic patients with cataracts by <t> Quantibody </t> Custom Array.
Raybio Quantibody Custom Array, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+arrays/pmc07058430-69-7-11?v=RayBiotech+inc
Average 90 stars, based on 1 article reviews
raybio quantibody custom array - by Bioz Stars, 2026-07
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cytometrin bead array soluble protein master buffer kit  (Becton Dickinson)
90
Becton Dickinson cytometrin bead array soluble protein master buffer kit
The expression levels of cytokines marked changes in high myopic patients with cataracts and non-myopic patients with cataracts by <t> Quantibody </t> Custom Array.
Cytometrin Bead Array Soluble Protein Master Buffer Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) PIP array spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.

Journal: Science signaling

Article Title: Hippo and PI5P4K signaling intersect to control the transcriptional activation of YAP

doi: 10.1126/scisignal.ado6266

Figure Lengend Snippet: (A) MOB1 phosphorylation in WT and ⍺β DKO with or without re-expression of PI5P4K⍺ (⍺ WT) or kinase-dead PI5P4K⍺ (⍺ KD) MEFs. Quantified Western blot results are presented as P-MOB1/MOB1 ratio (right), average of 3 independent experiments (one way ANOVA, followed by Welch’s correction). (B) Co-immunoprecipitation of over-expressed MOB1SFB and endogenous LATS in WT and ⍺β DKO HEK 293A cells. Quantified Western blot results are represented as LATS1/MOB1SFB ratio averaged over 3 independent experiments (right, Student’s t-test, **p<0.01). (C) PIP array spotted with eight phosphoinositides at decreasing concentrations and probed with purified human MOB1MYC. Representative of 3 independent experiments. (D) PIP strip probed with purified human MOB1WT or a truncated ΔN33–216 MOB1 (MOB1∆N-term). MOB proteins were immunoblotted with an antibody against His-Tag. Representative of 3 independent experiments. (E) Computed binding energies of 9 positions from PyRx blind docking of PI5P and PI4,5P2 (16:0/16:0) to MOB1 (p=0.0001 by unpaired t-test). (F) RMSD over 50ns Amber17 MD simulations of MOB1 bound to either PI5P (green) or PI4,5P2 (blue) using the lowest-energy docked structures as the initial state. (G) RMSF of the indicated N-terminal MOB1 residues over the entire MD simulation. (H) RMSF of the phosphoinositide atoms over the MD simulation. (I) Representative structure from PyRx docking showing interaction of Arg8 in MOB1 with the PI5P headgroup. The N-terminus of MOB1 is indicated by a yellow sphere.

Article Snippet: PIP Array PIP Array (P6100, Echelon) were incubated with recombinant human MOB1B C-MYC/DDK (TP306337, OriGene) and processed per the manufacturer’s instruction.

Techniques: Expressing, Western Blot, Immunoprecipitation, Purification, Stripping Membranes, Binding Assay

The expression levels of cytokines marked changes in high myopic patients with cataracts and non-myopic patients with cataracts by Quantibody Custom Array.

Journal: Molecular Vision

Article Title: The expression of cytokines in aqueous humor of high myopic patients with cataracts

doi:

Figure Lengend Snippet: The expression levels of cytokines marked changes in high myopic patients with cataracts and non-myopic patients with cataracts by Quantibody Custom Array.

Article Snippet: To reduce errors and improve accuracy, a quantitative protein detection chip RayBio Quantibody Custom Array (product QAH-CUST; RayBio; RayBiotech, Norcross, GA) was used for the verification of the cytokines of interest in the confirmatory stage.

Techniques: Expressing

The differences in the fluorescence of the cytokines with statistical significance in the Quantibody Custom Array. The higher the fluorescent brightness, the higher the concentration of the cytokine in the aqueous humor.

Journal: Molecular Vision

Article Title: The expression of cytokines in aqueous humor of high myopic patients with cataracts

doi:

Figure Lengend Snippet: The differences in the fluorescence of the cytokines with statistical significance in the Quantibody Custom Array. The higher the fluorescent brightness, the higher the concentration of the cytokine in the aqueous humor.

Article Snippet: To reduce errors and improve accuracy, a quantitative protein detection chip RayBio Quantibody Custom Array (product QAH-CUST; RayBio; RayBiotech, Norcross, GA) was used for the verification of the cytokines of interest in the confirmatory stage.

Techniques: Fluorescence, Concentration Assay